Multiple-Labeling of Oligonucleotide Probes for In Situ Hybridization.

In situ hybridization with oligonucleotide probes.

Equipment and reagents • Glass coverslips, 22x22mm • 6-well plates • 4% freshly made paraformaldehyde (Electron Microscopy Sciences) in PBS, pH 7.4 • Triton X-100 (Sigma) • Parafilm • Forceps, Dumont, GG (Electron Microscopy Sciences) • Avidin-DCS-Texas Red or –fluorescein (Vector) • Filter paper • Mounting medium (Molecular Probes) • Microscopy coverslide • Deionized formamide (Ambion) • Yeast...

Labeling of in situ hybridization DNA probes

Labeling fluorescence in situ hybridization probes for genomic targets.

Fluorescence in situ hybridization (FISH) requires nucleic acid probes, including deoxyribonucleic acid (DNA), ribonucleic acid (RNA), or nucleic acid analogs, labeled directly with fluorophores, or capable of indirect association with fluorophores. The nucleic acid provides the FISH assay with its specificity through complementary pairing of the probe nucleotides with nucleotides of the target...

Oligonucleotide probes for specific detection of Giardia lamblia cysts by fluorescent in situ hybridization.

AIMS Our study focused on the design of oligonucleotide probes and a suitable hybridization protocol that would allow rapid and specific identification of potentially viable cysts of the waterborne parasite Giardia lamblia. METHODS AND RESULTS Comparative analysis of ribosomal RNA (rRNA) sequences of Giardia lamblia and a number of closely and more distantly related species identified six reg...

OligoMiner: A rapid, flexible environment for the design of genome-scale oligonucleotide in situ hybridization probes